Review information of Reikan FoCal can be found here: https://www.dpreview.com/forums/thread/3211296

I have and would recommend Reikan FoCal software - see here: https://www.reikanfocal.com/

Thanks for all the suggestions and information - very much appreciated. I have had a couple of PMs from 'olsonview' and his comments along with the info provided here lead me to making a decision and ordering a Nikon CFI PLAN 10x/0.25NA 10.5mm WD objective for my project, along with an adapter and a step-down ring to enable me to mount the objective to the front filter thread on a Canon EF 70-200mm f/2.8 IS II USM used as a 'tube lens' to provide the required distance from the objective to the camera sensor and to focus the objective image on the sensor. This lens combination will then be attached to either a Canon 7D Mk-II or a Canon 5D Mk-IV body and mounted on a Cognisys StackShot to move the camera in extremely tiny increments and to take shots following each movement. The shots will then be 'stacked' in Heicon Focus 6.7 (see here: http://www.heliconsoft.com/heliconsoft-products/helicon-focus/) to create one final image with complete depth of field from front to back. The StackShot and a specimen mount will be attached to a solid board to help eliminate any possibilities of vibration (see photo of possible/example configuration below).

I am an ex Entomologist (used to undertake biological surveys and started the Cheshire Local Environmental Record Centre at Chester Zoo - see: http://www.record-lrc.co.uk). I would like to take fully in-focus (complete depth of field shots) of parts of insects and other invertebrates in order to produce images which can help others in identifying those creatures. I already have a Meiji EMZ-5TR trinocular head zoom stereo microscope and a Brunel SP500 trinocular compound microscope for entomological work.

Thanks once again for everyone's assistance.

Steve

Hi Everyone,

I'm new here but hope you can help me. I am a Canon user with a particular interest in macro-photography (mainly wildlife (insects etc.)) and now want to move beyond the scope of of 100mm macro and 65mm 1-5x macro lenses into the realm of 10x to 20x magnification. I intend to set up a macro-bench (chunk of wood) with a Stackshot and specimen holder attached and hope to attach a microscope 10x PLAN Infinity objective to a 'tube lens' and then a Canon body (either 7D Mk-II or 5D Mk-IV) to enable me to reach such degrees of magnification.

However, microscope objectives (good ones) tend not to be cheap and I was wondering if anyone uses such a set-up and if they can recommend good microscope obectives for what I am trying to achieve ??

Many thanks for any help/suggetions you can provide

Steve.

I can recommend this Codec - http://www.fastpictureviewer.com/codecs/

Steve