sippyjug104 Loc: Missouri

Jeff, thanks for dropping in.

sippyjug104 Loc: Missouri

Thanks, Randave2001. It's a bit difficult to see at first, however she has eight eyes. There are two at the very top and the ones on the sides are pairs of two. Some spiders, like the brown recluse, for example, have six eyes.

sippyjug104 Loc: Missouri

Earnest, thanks for viewing.

sippyjug104 Loc: Missouri

Mel, thanks for the feedback.

sippyjug104 Loc: Missouri

Thanks, Kpmac. There are plenty of orb weavers down your way and they are among the many good spiders.

sippyjug104 Loc: Missouri

inl, I'm confident that she would feel the same way about me. Actually, I feel the same way about me!

sippyjug104 Loc: Missouri

TreborLow, thanks for the kind reply. A microscope objective is designed for high magnifications. When we look at someone using a microscope, the objective nearly touches the slide being viewed. The slightest movement up or down brings the slide in or out of focus. Now, imagine putting a microscope objective on a camera for the same situation applies. Of course, a digital motorized focus rail controlled by a PC makes consistent precision moves of the camera practical. I have over 400 lbs. of mass with layers of high-density vibration isolation setting on the concrete in-ground basement floor to quell any transient vibration.

TreborLow

Dear Sippyjug,
I bought one of those microscope lenses, but only use it on extension tubes. Is there a guide to the spacing between a "regular" lens and the added microscope lens? I presume it reduces the DOF even more. Now I have a manual rail, so microns are beyond my reach. I am a Pentaxian, so quite a few older manual lenses to play with as well. My best shot so far is red thread going through a needle eye. Old 80mm lens with every passive extension tube I had! Hung my camera below a tripod, looking down to reduce vibrations. Used the microscope lens on some flat obects, including Lincoln, inside the his monument on the back of the bill!
Many years ago, I had access to an excellent professional microscope with a splitting system and took images of individual biological tissue cells. I guess that is where the interest began.
Thanks again,
Bob

sippyjug104 Loc: Missouri

Thanks, CPO. I've often thought the same thing.

Sylvias Loc: North Yorkshire England

Wonderful work Gary.

sippyjug104 Loc: Missouri

Bob, thanks for asking for I truly enjoy sharing what I do and how I do it.

There are essentially two types of microscope objectives. One is "finite" and the other is "infinite". Both perform equally well and both are highly used in microscopy.

The difference is that FINITE objectives focus on a point at a specific distance, typically 160 millimeters measured from the shoulder of the threads to the sensor of the camera.

INFINITE objectives do not bring the waves of light to a focus point and they require a secondary lens, referred to as a tube lens, to bring the objective into focus. This is typically rated at a 200mm lens. The advantage of this is that as the millimeter of the tube lens changes, so does the magnification produced by the objective.

If this is the type of objective that you have then 200mm from the camera sensor produces the rated magnification. mounting it on the end of a 100mm lens would produce half the rating and so on. Keep the aperture of the lens fully open and set it to infinity. Using lenses greater than 200mm produces more magnification accordingly. To use a camera lens as a tube lens, you will need to adapt the lens filter thread size down to that of the microscope thread size (most often the RMS thread size).

The AMSCOPE 4X Plan Achromatic Finite objective is a top-rated microscope objective used for macro photography that sells for $30.00. What macro lens could anyone buy for thirty dollars? If you are in the market for one, you can't go wrong with this one and it provides ample working distance to illuminate the subject.

https://amscope.com/products/4x-plan-achromatic-objective-lens-knurled-ring

This is the objective that I started with and I used a manual worm-screw focus rail. The NA (Numerical Aperture) of the 4X objective is 0.10 so the depth of field is 0.055 millimeters so the travel of the camera for a shot taken in a stack should be no greater than that.

Of course, if you are using it for magnified pictures of stamps or other such flat items, then simply move the camera until a sharp focus is obtained for no focus stacking would be required.

sippyjug104 Loc: Missouri

Thanks, Sylvia. I appreciate your viewing.

47greyfox Loc: on the edge of the Colorado front range

Excellent! Another beauty!

sippyjug104 Loc: Missouri

Thanks ever so much, 47GreyFox.

Bubalola Loc: Big Apple, NY

Excellent outcome, Gary!