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Odd artifacts in Lightroom exports
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Oct 4, 2022 12:35:04   #
Robertl594 Loc: Bloomfield Hills, Michigan and Nantucket
 
sippyjug104 wrote:
Robert, This is looking really good and to answer your question about the step distance of the 5X mitty infinity objective, the step distance is dependent on the depth of field produced by the optic. For microscope objectives, it becomes quite easy to determine for the objective are listed with their "NA" (numerical aperture) which is a guide to what the DOF will be at the rated magnification.

It is a fixed value and it will not change as it would with a lens with a variable aperture so your step distance will remain constant (although you can always shoot in smaller steps, but not larger ones).
Robert, This is looking really good and to answer ... (show quote)


Excellent! Thank you.

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Oct 4, 2022 14:11:20   #
DirtFarmer Loc: Escaped from the NYC area, back to MA
 
Robertl594 wrote:
Have not used PNG for photos, just logos. Do you? Benefits?...


I use jpg for photos generally. I use png for photos with transparency when it's required, not otherwise. But the png compression is lossless. https://en.wikipedia.org/wiki/Portable_Network_Graphics#Compression

I did a study of jpgs to see what sort of problems are created by the compression. A synopsis of the study is here and contains a pointer to the full document (14 pages). Basically I don't see any real problems with jpgs for normal use as long as the compression used is reasonable. The compression level can generally be chosen when the file is written but different programs use different numbers to control the compression so you have to know just which number to use for your program.

I invite you to check for yourself the effect of compression. I find it difficult to compare two images side by side, but if you overlay them and alternately view one, then the other using a blink test you can see the effects much more easily. There are several ways to do a blink test. One way is to load the two images to be compared into Photoshop as separate layers, then just turn the top layer on and off. You can zoom in to see the effect on small areas, right down to pixel level. You will see differences, but the higher compression images will, as expected, show larger differences. Whether the differences are enough to produce a noticeable change in the image will be up to the individual viewer.

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Oct 4, 2022 14:23:21   #
DirtFarmer Loc: Escaped from the NYC area, back to MA
 
CHG_CANON wrote:
Like lots of things, here's a 'solution' with no purpose for a problem with no definition.


I only mentioned png files because the OP didn't like lossy compression. The only time I use them is when I need transparency.*

* I create plots using png because I have not yet figured out how to add a good color space to a jpg in Python when saving a graph bitmap as an image in my graphing program. Also, I use Pixea on my MacBook because it handles files within a folder in a way similar to IrfanView (Windows only) which I have used for a couple decades. But it doesn't print worth a damn. Preview handles printing well but doesn't handle files the way I'm used to. So jpgs go to Pixea and pngs go to Preview as the default viewer. If I want to print a jpg I can 'open with' Preview, but it's not as simple as using the default viewer.

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Oct 4, 2022 14:56:51   #
Robertl594 Loc: Bloomfield Hills, Michigan and Nantucket
 
Thanks for your in-depth reply. Good information, but I like to keep it simple and easy. I default to the easiest way to get lossless images. The only time I use jpegs is when I am posting something to the web. They do not degrade when opened on the web as they are not re-saved. When working on images, I use RAW and TIFFs. PNG for logos.

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Oct 4, 2022 16:06:52   #
joecichjr Loc: Chicago S. Suburbs, Illinois, USA
 
Robertl594 wrote:
Next attempt is attached. Adjusted lighting. 224 Images spanning approx 1/8th of an inch of depth = .025mm each slice.


An eerie masterpiece 🕷️🕷️🕷️🕷️🕷️

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Oct 4, 2022 16:21:23   #
joecichjr Loc: Chicago S. Suburbs, Illinois, USA
 
Robertl594 wrote:
I have returned home. I did a new stack of new photos of the head of a bumble bee, exported using LR export to tiffs. No issues at all. Hopefully those artifacts were just a one off issue. I will keep you posted. Thank you for your suggestions.

Now I need to work on cleaning subject and adjusting lighting.

I would like to send a huge THANK YOU to Sippyjug104 who has provided me with patient, kind, diligent support and assistance in helping me transform my focus stack rig and technique over the last several weeks. Gary, I could not have done this without your help. Your expertise and knowledge is phenomenal. I hope I can return your kindness somehow, someway, someday soon. Now, we are having fun!

This is why I love UHH! There are some very generous, kind, competent people here that have the knowledge, experience, kindness and willingness to help the novice to pro photographers, advance our craft.
I have returned home. I did a new stack of new pho... (show quote)


Mindbogglingly impressive
🥇🥇⭐🥇🥇

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Oct 4, 2022 16:38:53   #
Robertl594 Loc: Bloomfield Hills, Michigan and Nantucket
 
joecichjr wrote:
Mindbogglingly impressive
🥇🥇⭐🥇🥇


Thank you so much!!!

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