Grahame wrote:
From my reading and personal experience on this the rail is best for very small subjects, and the focus barrel best for larger subjects.
Reasons appear to be that the stacking software can handle differences in magnification (using fixed camera position) better than differences in perspective (using rail/moving camera).
When using the lens barrel, if you are lucky enough to have a macro lens with 'serrations' on the focus ring these are excellent for controlling step distance.
This was done adjusting the focus "barrel" . . .
I've done it both ways with similar results - setup time is much faster without the rail.