I never used the original version because I'm not a fan of push/pull zooms. Some people love em... Some people are like me, and don't.
Push/pull zooms are quite fast to use. I've noticed over the years that the original 100-400 is particularly popular among air show photographers and folks shooting birds in flight... where the really fast zoom action seems a benefit. Some folks refer to the push/pull 100-400 as a "dust pump" and feel it leads to more sensor cleanings. I don't know if that's true or not. But it was another concern I had that made the lens less than attractive to me. Still, I have seen a lot of people use it to make excellent shots.... and the prices are sure good right now. It's still available new some places, for about $500 less than it sold for before the Mark II was introduced. Note that the original doesn't play well with filters.... Over the years I've seen a lot of people having concerns about "soft" images, who were surprised how much sharper the lens was as soon as they removed the "protection" filter they had on it. That seems to happen regardless of filter quality.
The Mark II is a more standard 2-ring zoom design. I got one recently and have made a few thousand shots with it (on 7DII). Seems quite good. The guys over at Lensrentals.com took one apart to see what it looks like inside (they love to do that). They commented that it was one of the best-built lenses they'd ever disassembled... certainly the best zoom, rivaling some super tele primes that cost close to $10,000. You can read their thoughts and see the guts of the lens on their blog, if you wish (
https://www.lensrentals.com/blog/2015/02/canon-100-400-is-l-mk-ii-teardown-best-built-lens-ever/).
The Mark II has 3 to 4-stop Image Stabilization. The original's IS was rated as 2 to 3-stop. I've got other lenses with both types of IS that I've used for a lot longer and have to say that either is helpful... the newer type of IS is just a little more helpful. A lot still depends upon good technique, but you can get more sharp shots at slow shutter speeds. The original 100-400 seems to have the earlier type that has to be turned off when on a tripod. I think the Mark II's IS will self-detect and turn itself off (other lenses of this type, I never turn off the IS). The 100-400 II is the least expensive Canon lens to have "Mode 3" IS... you have to spend at least $7000 to get any other EF lens with it. This is "instant IS" that only activates
during the exposure.... which suggests that the IS is extremely fast-acting. It's also found on Mark II version EF 300/2.8 IS, 400/2.8 IS, 500/4 IS etc. I have not used "Mode 3" yet... really not sure I will have much reason to. But it's there if I need it.
Both lenses are relatively large and heavy... about 3.5 lb. I shot for over 12 hours with mine last Sunday, all handheld and almost non-stop, and my arm is still a bit sore. For comparison, the EF 300/4 IS and various Canon 70-200/2.8 lenses are about 2.5 lb. They also are a bit smaller, though the 100-400mm does retract quite a bit for storage (the 300/4 and 70-200/2.8s are IF lenses, so don't retract at all).
The 100-400 II is rather large diameter. My hands are rather average size, but got tired and cramped up after handholding the lens for 12 hours.
The larger front ring of the Mk II is the zoom ring, and is where it feels most natural to handhold - "cradle" the lens with the left hand - while in use. (Most other Canon zooms, the rear ring is the zoom, while the front is focus.)
The Mark II is very close focusing. Used to be that my EF 300/4 IS was my closest-focusing long tele (i.e. my "poisonous snake" lens). For many years the 300/4 has been the closest-focusing, with higher magnification of any Canon lens longer than 200mm... except now for the 100-400 II. Should be handy for close-ups of small subjects such as butterflies etc. (or rattlesnakes!)
I use Arca-Swiss quick release system with my tripods and monopods. The 100-400 II's tripod mounting foot doesn't lend itself to an A-S mounting plate (plus seems a little risky design to me). There are at least three companies manufacturing replacement feet for the lens, with built-in Arca-Swiss dovetails... Really Right Stuff, Kirk Photo and Hejnar Photo. I ended up installing the latter, which seems excellent and more secure than the original. It also gives a bit bigger grip "handle" to carry the lens.
I also installed a LensCoat, which I've had on some other large lenses for as long as fifteen years and really like. However, on the 100-400 II the neoprene cover has a whole lot more small pieces and I'm not sure I care for it. I may try using some double-stick tape to better secure it in a few places. It does tone down the noticeable and intimidating "big white" lens a bit.
Both 100-400 are
not IF or "internal focusing" lenses... That means they extend in length considerably when zoomed. This changes their balance a bit if using them on a tripod or monopod... it's most noticeable (but manageable) if using them on a gimbal mount.
I think both also are varifocal zooms. I know the Mk II is. This is fairly common among modern auto focus zooms, and simply means they don't maintain focus when zoomed (parfocal zooms do, but are more complex. critical to calibrate and tend to be more expensive). This is most noticeable at close distances with the 100-400 II... But also isn't any problem at all when using AI Servo focus AF mode, since that will immediately correct for any loss of focus (so fast you won't notice on 7DII... other cameras may vary). If using One Shot AF, one needs to remember to re-focus after zooming any varifocal lens.
Both lenses use 77mm filters and are relatively "good light" f4.5-5.6 (a high-ISO capable camera will offset this). The Mark II maintains f5 up close to 300mm.
The hood supplied with the 100-400 II has a little sliding "door" on the under-side, to give easier access to a rotating filter such as a Circular Polarizer, so it's not necessary to remove the hood to adjust the filter. Haven't used that yet (the LensCoat covers it) and not sure if I will very much. Long teles like this I just don't tend to use with filters very often.
Overall, I'm happy with the 100-400mm Mk II. It seems an impressively capable lens, though I'm still learning to use and get accustomed to it.