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Dark Lines on Stack
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Mar 27, 2016 07:02:43   #
PaulBa Loc: Cardiff, Wales
 
A little help and advice please. Can anyone tell me what the dark lines are on the stack I’ve attached please.

I’ve only recently bought the MP-E65 and this is my third stack. The others had dark backgrounds although I did notice a few line across a lighter part of one of them which I removed in Lightroom and thought no more of it. However in this stack they appear to everywhere.
Any help and advice will be gratefully received.

Canon 6D at ISO 100, MP-E65 (set at 1:3), 1/180-sec at f/14, speedlight set at 1/8.
stack of 77 (Zerene Stacker) at 90um steps

Apologies for the quality of the photograph, I’m finding this lens enjoyable but difficult to use (i think it’s all in the lighting).

3rd Stack
3rd Stack...
(Download)

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Mar 27, 2016 09:22:08   #
oldtigger Loc: Roanoke Virginia-USA
 
what is it?

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Mar 27, 2016 09:42:50   #
tinusbum Loc: east texas
 
might be shadows,my highest fstop so far is f9 with the mpe65. lighting is the key with that lens,i'm still trying to learn it.

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Mar 27, 2016 09:51:08   #
PaulBa Loc: Cardiff, Wales
 
oldtigger wrote:
what is it?
Sorry but I’ve no idea.
I’ve sent a copy to my brother (a retired science teacher), he’s pretty good at identifying some of the odd things i photograph. It’s about 5mm long and has been cocooned in my conservatory for a couple of weeks, but has only started emerging during the last couple of days.

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Mar 27, 2016 09:58:35   #
PaulBa Loc: Cardiff, Wales
 
tinusbum wrote:
might be shadows,my highest fstop so far is f9 with the mpe65. lighting is the key with that lens,i'm still trying to learn it.
Thanks,

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Mar 27, 2016 10:08:59   #
LoneRangeFinder Loc: Left field
 
I checked the download and the pattern of this line of dark blotches appears to repeat though-out the image in varying degrees of density. There is a second (different) repeating pattern toward the bottom and right. What I would like to see is a few of the individual exposures used to make this stack. Has this occurred only when using flash?

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Mar 27, 2016 10:29:55   #
PaulBa Loc: Cardiff, Wales
 
LoneRangeFinder wrote:
I checked the download and the pattern of this line of dark blotches appears to repeat though-out the image in varying degrees of density. There is a second (different) repeating pattern toward the bottom and right. What I would like to see is a few of the individual exposures used to make this stack. Has this occurred only when using flash?
Thanks LoneRangeFinder
I’ve attached two of the original pictures (reformatted from RAW to JPEG).
The shots were taken “on location”. the specimen was about six feet off the ground so I had my camera and flash on different, fully extended tripods, it took me ages to set up (but then that is half the fun).
I had a few lines on one of my other MP-E65 stacks although I remember having this issue once with my 100mm macro lens although nowhere severe as this one. The majority of my limited macro work has been with dark backgrounds which may be why I’ve not noticed this before.
Thanks for taking the time.


(Download)


(Download)

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Mar 27, 2016 10:45:37   #
LoneRangeFinder Loc: Left field
 
PaulBa wrote:
I had a few lines on one of my other MP-E65 stacks although I remember having this issue once with my 100mm macro lens although nowhere severe as this one. The majority of my limited macro work has been with dark backgrounds which may be why I’ve not noticed this before.
What is puzzling is that the precise pattern repeats-- but in a random pattern.
I don't see this on the individual shot-- although I do see a few individual spots, so perhaps the stacking created the "line". We may never know exactly what caused this.

So solution? I would suggest a sensor cleaning & opening up to f/5.6 or f/8. Will that eliminate this? Not sure. Good luck & let us know if you discover the "cure"

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Mar 27, 2016 10:47:36   #
EnglishBrenda Loc: Kent, England
 
I wonder if those black lines could be marks, perhaps from the sensor, that are repeated and repeated throughout the stacking in slightly different positions.

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Mar 27, 2016 11:29:08   #
naturepics43 Loc: Hocking Co. Ohio - USA
 
Definitely caused by dirt on your sensor. Two solutions, clean sensor or clone out in PP. (That's what I do if there are only a few close to the edges)

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Mar 27, 2016 11:41:02   #
naturepics43 Loc: Hocking Co. Ohio - USA
 
LoneRangeFinder wrote:
What is puzzling is that the precise pattern repeats-- but in a random pattern.
I don't see this on the individual shot-- although I do see a few individual spots, so perhaps the stacking created the "line". We may never know exactly what caused this.
I just viewed the first single frame. LOTS of dirt spots on sensor. F 14 will make them more visible. ZS does several things when stacking a set of images. One is "scaling" which changes the size of individual frames to make things line up. Another is "rotation". That's what is causing random patterns. ZS can also "shift x & shift y" during the stacking process. All done in the background.

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Mar 27, 2016 12:25:23   #
LoneRangeFinder Loc: Left field
 
naturepics43 wrote:
I just viewed the first single frame. LOTS of dirt spots on sensor. F 14 will make them more visible. ZS does several things when stacking a set of images. One is "scaling" which changes the size of individual frames to make things line up. Another is "rotation". That's what is causing random patterns. ZS can also "shift x & shift y" during the stacking process. All done in the background.
Thanks. That is what I suspected--but did not know for certain. The clue seemed to be that the object was actually not a line but a series of spots. Most of my stacking knowledge is "experiential" and since I keep a clean sensor and stack at f/5.6 to f/8, those were two variables that I noticed.

FWIW, I'm also relatively new with Zerene-- having started with Helicon. Helicon, in my opinion, has a much more difficult time with scaling.

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Mar 27, 2016 12:31:13   #
PaulBa Loc: Cardiff, Wales
 
LoneRangeFinder wrote:
I would suggest a sensor cleaning & opening up to f/5.6 or f/8. Will that eliminate this? Not sure. Good luck & let us know if you discover the "cure"
Thanks LoneRangeFinder. I’ll get the sensor cleaned.

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Mar 27, 2016 12:34:36   #
PaulBa Loc: Cardiff, Wales
 
naturepics43 wrote:
I just viewed the first single frame. LOTS of dirt spots on sensor. F 14 will make them more visible. ZS does several things when stacking a set of images. One is "scaling" which changes the size of individual frames to make things line up. Another is "rotation". That's what is causing random patterns. ZS can also "shift x & shift y" during the stacking process. All done in the background.
Thanks for taking the time to look at the original naturepics43.
I’ll get the sensor cleaned.

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Mar 27, 2016 13:44:12   #
Macronaut Loc: Redondo Beach,Ca.
 
naturepics43 wrote:
Definitely caused by dirt on your sensor. Two solutions, clean sensor or clone out in PP. (That's what I do if there are only a few close to the edges)
This is what I think too. It seems that this was about a 50 image stack.

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