Rome: the Pantheon, amazing, my favorite. Agree with Orvietto, pick up some great wine. Florence is nice, but I prefer Siena (bucket list-watch a Palio at the bleachers at San Martino curve). If driving to Venice, check out Bologna on the way.

Enjoy the food. Have a prosciutto and melone to start, a tartufo nero for dessert (ice cream - vanilla ice cream golf ball surrounded by a hemisphere of chocolate ice cream, with hard chocolate shell covered with powdered chocolate).

Skip the tripod, too much hassle for too little use. Leave NOTHING in the car, and, when parked, the glove box open to show nothing inside.

Repleo. You have provided a terrific overview and advice. I have been to most of those places, as well, and agree with your assessment totally. I would add that of the walkway and scenic point that you and Nunyaman mentioned, goes from Manarola to Corniglia on the ocean side. You can walk to Corniglia and take the train back or just walk at least to the suspension bridge and walk back to Manarola (preferred?). Many great scenic and plant shots.

Nunyaman, I would skip the tripod and consider bringing only the 24-70mm, 15mm and 70-200mm. You could keep the 70-200 on the 80D with an FFE of 112-320mm, it will be plenty long enough. Then I'd switch between the 15mm (interior and some scenic) and the 24-70mm for over 1/2 of the shots. By the way, I took several shots from the Manarola scenic point using 35-60mm FFE.

For a 21 day trip that I took to Italy (Venice, Florence, Rome, Radda, Cinque Terra, San Margarita/Lake Coma). I took 1897 keepers and of those: 526 were @ 24-34mm, 232 @ 35-49mm, 222 @ 50-74mm, 422 @ 75-105mm, 495 @ > 105mm.

Quick list of other locations not mentioned by the terrific ones that Repleo suggested

Regarding tripod or monopod and night photography, remember, at night there are less people about and in Venice you can rest your camera on a bridge wall, or iron fence and you can always find a wall or corner of a building to press against to get a night shot, so no need for a tripod. Take a non stretch piece of parachute cord with you. Make this a reverse tripod.

You tie a loop for your foot and another loop at the other end for your lens, placed near where it connects to the camera body. Now stretch this tight and hold your breath to take a long exposure at night and take a burst of shots too. You will almost always find a few perfectly still images in and burst, using the string. It helps to steady out any movement as you pull up on the string to keep things steady.

With a tripod you sort of push down to get it to be steady but the string, you pull up. When finished just roll up the cord and keep it in your pocket. Much better for steading a shot than not using anything at all, when you don't have a tripod or monopod to use.

We are headed to Italy for some quick R&R in mid November. 3 Days near Rome, 4 in Tuscany area and 3 near Venice. Have rental car and would love any info on landscape photo ops or should not miss areas. We have no real itinerary other than hotels and flights are booked. My wife will carry her Canon d80 and I will carry my EOS R. I have multiple lens but will narrow it down to the 15mm F/2.4, EF 24-70mm F/2.8L II, and most likely one of the mid zooms. One consideration is visiting the Manarola scenic viewpoint. if so, how much zoom would I need to get a good shot of the marina and buildings on the hill? I have a EF 70-200mm F2.8L IS II, EF 70-300mm F4-5.6 IS, and the monster weight 150-600 5-6.3 Sports DG OS HSM lens but that thing is a little heavy. I do have a good carbon tripod and mono pod. I assume I can use those in most areas. Thanks for any input from the group.