Philodendron Leaf - Backside Breathing Pores
Feb 8, 2020 14:46:38 #
This is the backside of a Philodendron leaf from a plant that was given to me at a hospital stay ten years ago. To the unaided eye the leaf appears to be smooth however when viewed under magnification it is quite anything but smooth.
I staged this session with a 10X Plan Infinity microscope objective mounted on a bellows with a reverse mounted Raynox DCR-150 used as the tube lens. Illumination is diffused LED constant lighting and I believe that this shows the breathing mechanism of the plant much better than the previous tiny green leaf that I brought in from the backyard.
As always, thanks in advance to all who view and for your comments, suggestions, questions and critique.
I staged this session with a 10X Plan Infinity microscope objective mounted on a bellows with a reverse mounted Raynox DCR-150 used as the tube lens. Illumination is diffused LED constant lighting and I believe that this shows the breathing mechanism of the plant much better than the previous tiny green leaf that I brought in from the backyard.
As always, thanks in advance to all who view and for your comments, suggestions, questions and critique.
Feb 8, 2020 16:16:19 #
Feb 8, 2020 16:29:25 #
I see stomates! They can open or close. These I suppose are closed. Still ... they are stomates.
This was fun!
This was fun!
Feb 8, 2020 16:48:34 #
Mark Sturtevant wrote:
I see stomates! They can open or close. These I suppose are closed. Still ... they are stomates.
This was fun!
This was fun!
Mark, I found it quite amazing at how smooth the leaf appeared to be on both sides. Of course the front was a darker green than the back side yet smooth the same. When I looked at it under magnification I could see the "fish lips" (stomates) that you had mentioned. I suspect that they had closed due to the trauma of me cutting a small piece of the leaf off about the size of my thumbnail for staging.
Feb 8, 2020 16:50:15 #
kpmac wrote:
Maybe could be used as a texture image, sippy.
Thanks, kpmac. Mark gave me a challenge to see if I could find any of the leafs breathing holes.
Feb 8, 2020 16:50:26 #
Feb 8, 2020 17:01:45 #
Blenheim Orange wrote:
Good stuff there. Very nice. Leaves are amazing little machines.
Mike
Mike
Thanks Mike, there is a lot more going on "than meets the eye" as it is often said.
Feb 8, 2020 18:25:41 #
Feb 8, 2020 18:36:58 #
EnglishBrenda wrote:
Thats it Gary, well done.
Brenda, these are very, very small. I mean like real small. This leaf looked to be smooth of both sides and I cranked up the magnification until I could see the pores starting to appear. I set the travel distance of the camera to 5-microns and stacked about 250 images on this one.
Like using any microscope, the higher the magnification the closer the tip of the objective is to the subject which makes lighting a challenge. Most microscope work is done with glass slides where the lighting is aimed through it into the tip of the objective.
Feb 9, 2020 05:27:16 #
sippyjug104 wrote:
Brenda, these are very, very small. I mean like r... (show quote)
Then you did well to find them Gary. I have been reading about stomata since your first post and there seems to be quite a variety of types and numbers on different leaves. Also, floating leaves have stoma on top of the leaf only and, of course, immersed leaves don't need them at all as there is no need to conserve water. This subject is worth a study in its own right. There are some Youtube videos on how to take a piece of thin 'skin' from the leaf surface and dye it before looking through the microscope lens.
Feb 9, 2020 09:41:17 #
EnglishBrenda wrote:
Then you did well to find them Gary. I have been r... (show quote)
Brenda, this is how I attach a microscope objective to serve as the camera's lens. The microscope objective is mounted onto a 'reverse' mounted 208mm focal length Raynox lens with extension tubes so that Raynox lens focuses to infinity. I use a bellows in place of the extension tubes at times also.
Feb 9, 2020 09:45:55 #
sippyjug104 wrote:
Brenda, this is how I attach a microscope objective to serve as the camera's lens. The microscope objective is mounted onto a 'reverse' mounted 208mm focal length Raynox lens with extension tubes so that Raynox lens focuses to infinity. I use a bellows in place of the extension tubes at times also.
It strikes me there is a lot of knowledge and skill in what you do, all well above my endeavours but I enjoy following your successes and techniques and am full of admiration.
Feb 9, 2020 10:57:13 #
EnglishBrenda wrote:
It strikes me there is a lot of knowledge and skill in what you do, all well above my endeavours but I enjoy following your successes and techniques and am full of admiration.
Brenda, I am humbled by your words and you played a large part in what has become my passion and I think of you as one of my mentors.
Feb 9, 2020 13:50:51 #
Ahhhhh..........the thrill of exploration..................motor on my friend......
Feb 9, 2020 14:04:26 #
photosbytw wrote:
Ahhhhh..........the thrill of exploration..................motor on my friend......
I have found that 99% of all my problems can be solved by money, hard work and ingenuity -- and for the other 1% there's Vodka..!
If some of my images are blurry at times...now you'll know why.
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