CEJ wrote:
I have one macro lens an AF-S Micro NIKKOR 85mm 1:3.5 ED, looking to shot down to 1mm in size saw as small as an aphid, in a studio setting with a light box. D200 back.
The Nikkor 85mm f/3.5 Micro AFS lens is a "G" version, which requires electrical connections to the camera in order to set the aperture. It will not work with a bellows, but will work with extension tubes if they have the electrical contacts.
However, if you want to frame an object that is 1mm in size, and have it cover most the frame, there is a huge problem! Your cameras have APS-C, aka "DX", sensors that are roughly 24mm wide and 16 mm high. To project a 1mm object to cover 10 of the 16mm of sensor height means a 10:1 magnification. That is not trivial.
Your 85mm f/3.5 will focus down to 2.8 centimeters, or just over 1 inch from the object. At that distance it provides 1:1 magnification. There are two things to realize... getting closer by use of more extension is not boing to help, and you will not get 10:1 magnification from that lens.
I suggest you consider using a bellows and one or more appropriate lenses. For 10:1 look at microscope objectives (and the necessary adapters needed to use them). Objectives made by Unitron and Nikon do not require chromatic correction in the ocular, so they make good camera lenses. Get objectives marked as "M" or "Plan", which are meant to be used with metallurgical microscopes. The ones marked as something like "160/-" are finite tube designs. The "160" is the tube length in mm, the "-" means no slip cover. If it is marked with an infinity symbol, "oo/-" then it will need to be in front of a "relay lens". The relay lens can be an older AiS Nikkor 200mm f/4 (available on eBay for less than $50). Low cost used Unitron objectives in 2.5x, 4x and 10x can be found on eBay, but make sure the front element is not damaged. Nikon lenses are more expensive, but various CF and CFI lenses can be found for less than $150 new.
A suitable bellows would be a used Vivitar with focusing rails. These are available on eBay for $30 and the focusing rail the same or less. The bellows is a T-mount. It requires adapters to the camera and the lens, but you can use just about any make of lens too. It also will require a short extenstion tube between the camera and the bellows.
That isn't the end of this adventure though... because at 10x magnification you do not get enough depth of field to work with! With an f/stop of f/11, at 10x magnification, the depth of field is something like 0.3mm. It's 1.3mm at f/22. All pretty critical. And the solution (with bugs that don't move) is focus stacking.
There are several programs, some a no cost, that will do a good job of focus stacking. Except that taking the stack is very very tedious! You might want 100, or even more shots to combine for a single picture. The answer is not inexpensive either. Take a look at
StackShot -- Automatic Focus StackingIt isn't inexpensive though, the focusing rail plus controller costs $550 and it's easy to add a couple hundred extra for accessories.